Dietary Fibre Testing Lab in India

If your product carries a dietary fibre declaration or a high-fibre claim, FSSAI requires it to be substantiated by NABL-accredited lab results. Auriga Research provides the full AOAC dietary fibre testing suite — covering conventional and novel fibres including inulin, fructo-oligosaccharides (FOS), polydextrose, resistant maltodextrin, and resistant starch.

Our scope covers total dietary fibre (TDF), soluble dietary fibre (SDF), and insoluble dietary fibre (IDF) by enzymatic-gravimetric methods (AOAC 985.29, 991.43), and the newer enzymatic-HPLC methods (AOAC 2009.01, 2011.25, 2017.16) that correctly recover the low-molecular-weight soluble fibres that the older Prosky method misses. Specialty add-ons cover beta-glucan in oats and barley (AOAC 995.16 / 992.28), inulin and FOS (AOAC 997.08 / 999.03), and resistant starch (AOAC 2002.02).

Backed by the Arbro Group's analytical heritage — Arbro Lab since 1990, Auriga Research since 2007 — with NABL ISO/IEC 17025 accreditation, our reports are issued against FSSAI nutritional labelling thresholds, the FSS (Advertising & Claims) Regulations 2018 limits for "Source of fibre" and "High in fibre" claims, Codex Alimentarius CAC/GL 2-1985, and the EU 1169/2011 fibre declaration framework. Reports are accepted by FSSAI licensing officers, APEDA Export Inspection Agencies, EIC, and international buyers.

Dietary Fibre Fractions Quantified

Total Dietary Fibre (TDF)

Sum of soluble and insoluble fractions. The figure declared in the FSSAI nutrition information panel and used for "Source of" and "High in" fibre claims.

Soluble Dietary Fibre (SDF)

Beta-glucan, pectin, gums, mucilages, inulin, FOS, resistant maltodextrin, polydextrose. Linked to LDL cholesterol reduction and post-prandial glycaemic control.

Insoluble Dietary Fibre (IDF)

Cellulose, hemicellulose, lignin, resistant starch type 1 and 2. Supports gut transit, stool bulk, and bowel regularity.

Specialty add-ons: beta-glucan (AOAC 995.16 / 992.28), inulin and FOS (AOAC 997.08 / 999.03), resistant starch (AOAC 2002.02), pectin, and individual non-digestible oligosaccharides.

Methods Available

AOAC 985.29 (Prosky) — total dietary fibre, enzymatic-gravimetric
AOAC 991.42 / 993.19 — soluble and insoluble dietary fibre, separate determination
AOAC 991.43 — total, soluble, and insoluble dietary fibre in foods
AOAC 2009.01 (McCleary) — TDF including resistant starch and non-digestible oligosaccharides, enzymatic-HPLC
AOAC 2011.25 — integrated total dietary fibre with HPLC quantitation of low-molecular-weight soluble fibre
AOAC 2017.16 — rapid integrated total dietary fibre
AOAC 995.16 / 992.28 — mixed-linkage (1→3),(1→4)-beta-D-glucan in cereals and oats
AOAC 997.08 / 999.03 — fructans (inulin and FOS) by enzymatic-HPLC
AOAC 2002.02 — resistant starch in starch and starch-containing samples

Sample Matrices Accepted

Breakfast cereals, muesli, granola, and extruded snacks
Bakery — bread, biscuits, cookies, crackers, multigrain rusks
Whole grains, atta, dalia, oats, barley, millets, and pulses
Fruit, vegetable, and seed-based products (jams, purees, smoothies)
Plant-based meat analogues and high-protein vegetarian foods
Infant cereals and weaning foods (FSSAI Infant Nutrition standards)
Sports and clinical nutrition powders, bars, and shakes
Functional beverages containing inulin, FOS, polydextrose, resistant maltodextrin
Bakery premixes, fortified flours, and added-fibre ingredients
Pet food, animal feed, and aquafeed

Regulatory Thresholds for Fibre Claims

Claim	Solid food	Liquid food	Per 100 kcal
Source of fibre	≥ 3 g / 100 g	≥ 1.5 g / 100 mL	≥ 1.5 g / 100 kcal
High in fibre	≥ 6 g / 100 g	≥ 3 g / 100 mL	≥ 3 g / 100 kcal

Source: FSS (Advertising & Claims) Regulations 2018, Schedule I. The recommended dietary allowance for Indians is approximately 25-30 g of dietary fibre per day (ICMR-NIN 2020).

Applications

FSSAI nutrition information panel — declaring dietary fibre in the labelling block
Substantiating "Source of fibre" and "High in fibre" claims under FSS (Advertising & Claims) Regulations 2018
New product development for inulin-, FOS-, and resistant-starch-fortified foods
Reformulation of bakery and snack products for fibre enrichment
Export documentation — Codex CAC/GL 2-1985 and EU 1169/2011 fibre declarations
Functional food and nutraceutical positioning — prebiotic and gut-health products
Infant and clinical nutrition compositional verification
Quality control of fibre ingredients (inulin, polydextrose, psyllium, oat bran)
Shelf-life and process monitoring of dietary fibre retention during baking and extrusion

Who Needs This Testing

Packaged food and cereal brands declaring dietary fibre on their FSSAI nutrition information panel.
D2C and e-commerce brands making "Source of fibre" or "High in fibre" claims under the FSS (Advertising & Claims) Regulations 2018 — claims must be substantiated by an NABL-accredited lab result.
Food manufacturers using inulin, FOS, polydextrose, resistant maltodextrin, or resistant starch as functional fibre ingredients — these require the newer AOAC 2009.01 / 2011.25 methods.
Nutraceutical, health food, and prebiotic brands positioning products for gut health, microbiome, and digestive wellness benefits.
Infant food and weaning food manufacturers verifying fibre composition against FSSAI Foods for Infant Nutrition Regulations 2020.
Bakery, biscuit, and snack manufacturers reformulating for multigrain, fortified, and added-fibre product launches.
Exporters needing Codex CAC/GL 2-1985 and EU Regulation 1169/2011 dietary fibre declarations on labels for international markets.
Functional beverage brands launching inulin-enriched, FOS-fortified, or resistant-maltodextrin drinks where the fibre claim is the product's primary differentiator.

Why Auriga for Dietary Fibre Testing

Full AOAC method suite under one roof

AOAC 985.29 (Prosky), 991.43, 2009.01 (McCleary), 2011.25 (integrated TDF), and 2017.16 (rapid integrated TDF) on the same lab floor — we pick the method that fits your formulation, not the one we happen to have.

AOAC 2009.01 & 2011.25 for novel fibres

The newer integrated TDF methods correctly recover inulin, FOS, polydextrose, resistant maltodextrin, and resistant starch — fibres that the older AOAC 985.29 Prosky method misses because they pass through the 78% ethanol precipitation step. This is a genuine technical differentiator for brands using functional fibre ingredients.

Specialty fibre add-ons

Beta-glucan in oats and barley (AOAC 995.16 / 992.28), fructans / inulin / FOS (AOAC 997.08 / 999.03), and resistant starch (AOAC 2002.02) — accredited for the full functional-food fibre scope.

FSSAI claim substantiation

Reports map directly to the FSS (Advertising & Claims) Regulations 2018 thresholds (≥3 g / 100 g for "Source of fibre", ≥6 g / 100 g for "High in fibre") with explicit comparison against the on-pack claim.

Accepted for FSSAI, APEDA, EIC, and export documentation

NABL-accredited reports accepted by FSSAI licensing officers for label substantiation, APEDA and EIC for export dispatch certification, and international buyers requiring Codex CAC/GL 2-1985 or EU 1169/2011 compliance.

Arbro Group analytical heritage

Established analytical heritage through the Arbro Group (Arbro Lab since 1990, Auriga Research since 2007), with NABL ISO/IEC 17025 accreditation — the audit trail FSSAI, APEDA, marketplaces, and international buyers look for in a nutritional labelling partner.

How It Works

Get a Quote

Share your product type and the parameters you need tested. Your dedicated SPOC will confirm the testing scope, the applicable method, and the exact sample quantity required for your specific panel before you dispatch anything.

Send Your Sample

Dispatch your sample with a completed Test Request Form to the nearest Auriga lab. Each sample is individually bar coded and registered in YLIMS, Auriga's in-house Laboratory Information Management System, upon receipt. Testing begins within 24 hours of sample registration.

Testing and QA Review

Your sample is tested against the confirmed validated method by Auriga's scientific team. Every result passes through a formal internal QA review and sign-off before the report is generated.

Receive Your NABL Report

Your NABL-accredited test report is delivered digitally within the committed turnaround time. Reports carry Auriga's NABL accreditation under ISO/IEC 17025:2017 and are accepted by FSSAI, APEDA, EIC, and major international buyers. You can track your sample status in real time through YLIMS at any point in the process.

Turnaround Time

AOAC 985.29 total dietary fibre and AOAC 991.43 soluble/insoluble fractions are reported within 7-10 working days of sample receipt. AOAC 2009.01 and 2011.25 with HPLC quantitation of low-molecular-weight soluble fibre require 10-12 working days. Specialty fibre add-ons — beta-glucan, fructans (inulin / FOS), resistant starch — add 3-5 working days. Expedited reporting is available for time-critical FSSAI label and export submissions.

Related Services

Frequently Asked Questions

Which AOAC methods do you use for dietary fibre analysis?

We follow the full suite of AOAC enzymatic-gravimetric and enzymatic-HPLC methods. AOAC 985.29 (Prosky) and AOAC 991.43 are the workhorse methods for total, soluble, and insoluble dietary fibre in conventional foods. For products containing resistant starch, inulin, polydextrose, fructo-oligosaccharides, or resistant maltodextrin, we use AOAC 2009.01 (McCleary), AOAC 2011.25 (integrated total dietary fibre), or AOAC 2017.16 (rapid integrated TDF) which capture both high- and low-molecular-weight non-digestible carbohydrates that older Prosky methods miss. Reports cite the exact method used so labels and claims line up with the analytical approach.

What is the difference between soluble and insoluble dietary fibre?

Soluble fibre dissolves in water and forms a viscous gel during digestion — it slows gastric emptying, lowers post-prandial glucose, and binds bile acids to reduce LDL cholesterol. Sources include oats (beta-glucan), barley, legumes, psyllium, apples, citrus pectin, and inulin-rich foods. Insoluble fibre does not dissolve in water and adds bulk to stool, accelerating intestinal transit and supporting bowel regularity. Sources include wheat bran, whole grain cereals, nuts, seeds, and the structural cell wall of fruits and vegetables. Total dietary fibre (TDF) is the sum of both fractions and is what FSSAI nutrient information panels declare.

What dietary fibre level is required for FSSAI "source of fibre" and "high in fibre" claims?

Under the FSS (Advertising & Claims) Regulations 2018 and the FSS (Labelling and Display) Regulations 2020, a packaged food can declare "Source of fibre" only when it contains at least 3 g of dietary fibre per 100 g of solid product, or 1.5 g per 100 kcal. The "High in fibre" claim requires at least 6 g per 100 g of solid product, or 3 g per 100 kcal. Liquid products use 1.5 g and 3 g per 100 mL thresholds respectively. Our NABL-accredited report quantifies total dietary fibre against these thresholds so brands can substantiate the claim on pack.

How do you handle resistant starch, inulin, and other novel fibres?

The original Prosky method (AOAC 985.29) under-recovers low-molecular-weight soluble fibres such as inulin, fructo-oligosaccharides (FOS), polydextrose, resistant maltodextrin, and resistant starch type 4 because these compounds pass through the 78% ethanol precipitation step. For products formulated with these ingredients we recommend AOAC 2009.01 or AOAC 2011.25, which combine enzymatic digestion with HPLC quantitation of the soluble fibre fraction. This is the only analytically defensible approach for products labelling chicory inulin, Fibersol, Promitor, Litesse, or resistant starch as the fibre source.

How long does dietary fibre testing take?

Standard turnaround is 7-10 working days for AOAC 985.29 / 991.43 (total, soluble, and insoluble fractions). AOAC 2009.01 / 2011.25 require 10-12 working days because of the additional HPLC analysis. Sample quantity depends on the product category, product format, and the AOAC method selected — our team will recommend the appropriate quantity after reviewing your testing requirement. Expedited reporting is available for time-critical FSSAI label submissions.

Is the dietary fibre report acceptable for FSSAI nutritional labelling?

Yes. Auriga Research is NABL-accredited (ISO/IEC 17025:2017) and FSSAI-notified, and our scope includes dietary fibre testing under recognised AOAC methods. Reports are accepted by FSSAI licensing authorities for nutrition information panel substantiation, "source of fibre" and "high in fibre" claim verification, FSSAI Pre-Packaged Food labelling submissions, EIC export inspection certificates, and third-party retailer audits.

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