Delhi · Gurugram · Bangalore · Baddi · Bahadurgarh

Nutraceutical Stability Testing | ICH Q1A | NABL Accredited

Without analytically supported shelf-life data, a nutraceutical brand has no defence against FSSAI label-claim challenges, marketplace product complaints, or distributor returns near end of life. Backed by the Arbro Group's unbroken NABL accreditation since 2003, Auriga Research runs full accelerated and real-time stability studies for health supplements, dietary supplements, probiotics, and functional foods under ICH Q1A conditions.

Stability data is mandatory under the FSS (Health Supplements, Nutraceuticals, Food for Special Dietary Use, FSMP, Functional Food and Novel Food) Regulations 2016 Form B nutraceutical licence dossier. Our walk-in stability chambers operate at ICH Q1A(R2) Climate Zone IVa conditions for India — accelerated (40 °C / 75% RH), intermediate (30 °C / 65% RH), and long-term real-time (30 °C / 75% RH) — plus refrigerated 5 °C ± 3 °C chambers for fish oil, krill oil, and probiotics, and ICH Q1B photostability for vitamin and fat-soluble formulations.

Every chamber is on continuous temperature and humidity monitoring with deviation alarms and a full audit trail in YLIMS — the same monitoring evidence FSSAI assessors and international auditors look for.

Screening: 4–6 weeks | Accelerated: 7 months | Long-term: 12–36 months
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Stability Conditions & Parameters

Each parameter is mapped to its ICH condition or method so QA teams can match scope to FSSAI / Form B requirements at a glance.

ICH Q1A

Accelerated (40 °C / 75% RH)

Accelerated stability per ICH Q1A(R2) — 28-day screening or 6-month full study. Used for early shelf-life prediction and supplier qualification.

ICH Q1A

Intermediate (30 °C / 65% RH)

30 °C / 65% RH intermediate condition — triggered where significant change occurs in accelerated study or as required by jurisdiction.

ICH Q1A

Long-term (30 °C / 75% RH)

Long-term Zone IVa real-time condition for India — 12 to 36 months matching the claimed shelf life on the label.

5 °C ± 3 °C

Refrigerated Stability

5 °C ± 3 °C refrigerated condition for fish oil, krill oil, refrigerated probiotics, and live-microorganism supplements.

ICH Q1B

Photostability

ICH Q1B photostability test — confirmatory exposure to ICH Option 2 light source. Critical for vitamin C, B2, and fat-soluble vitamin photodegradation profiling.

HPLC

Vitamin Assay Trending

HPLC assay of declared vitamins at each stability time point — supports the 80% claim floor at end of shelf life per FSSAI 2016.

ICP-OES

Mineral Assay Trending

ICP-OES assay of declared minerals at each time point — minerals are typically stable but premix qualification still needs verification.

GC-FID

Omega Fatty Acid Trending

GC-FID for EPA / DHA / ALA at each time point — fish oil and omega supplements are particularly oxidation-prone and need active monitoring.

CFU

Probiotic Viability Trending

Strain-specific viable cell counts at each time point — typical probiotic stability shows 1–2 log loss across 24 months in optimised packaging.

KF Titration

Moisture Content

Karl Fischer moisture for capsules, tablets, powders, and sachets — moisture pickup during stability is the leading cause of probiotic and vitamin loss.

Microbial

Microbial Limits Trending

TPC, yeast and mould, pathogen testing at each time point — confirms preservative system efficacy across the full shelf life.

Physical

Physical Attributes

Appearance, colour, hardness, friability, disintegration, dissolution, pH, viscosity — supports tablet, capsule, sachet, and liquid SKU integrity.

How It Works

1

Get a Quote

Share your product type and the parameters you need tested. Your dedicated SPOC will confirm the testing scope, the applicable method, and the exact sample quantity required for your specific panel before you dispatch anything.

2

Send Your Sample

Dispatch your sample with a completed Test Request Form to the nearest Auriga lab. Each sample is individually bar coded and registered in YLIMS, Auriga's in-house Laboratory Information Management System, upon receipt. Testing begins within 24 hours of sample registration.

3

Testing and QA Review

Your sample is tested against the confirmed validated method by Auriga's scientific team. Every result passes through a formal internal QA review and sign-off before the report is generated.

4

Receive Your NABL Report

Your NABL-accredited test report is delivered digitally within the committed turnaround time. Reports carry Auriga's NABL accreditation under ISO/IEC 17025:2017 and are accepted by FSSAI under the Health Supplements Regulations 2016, BIS, and international buyers. You can track your sample status in real time through YLIMS at any point in the process.

Turnaround Time

Study Type Standard TAT Express
Screening accelerated (28 days) 4–6 weeks total On request
Full accelerated study (6 months at 40 °C / 75% RH) 7 months elapsed
Intermediate (30 °C / 65% RH) — triggered 6–12 months
Long-term real-time (12–36 months at 30 °C / 75% RH) 12–36 months elapsed
Refrigerated (5 °C ± 3 °C) for probiotics / fish oil 6–24 months elapsed
Photostability (ICH Q1B confirmatory) 4–6 weeks Available

Who Needs This Testing

  • Nutraceutical manufacturers preparing FSSAI Form B Nutraceutical Licence dossiers — stability data is a mandatory component of the submission.
  • D2C supplement and probiotic brands needing analytically supported "Best Before" or "Use By" dates for product launch and marketplace listing.
  • Brands extending shelf-life claims (12 to 24 months, 24 to 36 months) and needing real-time data to support the extension.
  • Manufacturers running supplier qualification on a new active-ingredient source, premix, or excipient base — accelerated stability screens predict the impact.
  • Probiotic and live-microorganism supplement brands needing CFU stability through the full shelf life to defend the label CFU claim.
  • Brands launching in new packaging formats (sachet vs blister, glass vs HDPE bottle, primary vs secondary moisture barrier) needing packaging-specific stability data.
  • Brands exporting to humid climate markets and needing Zone IVa data to support shelf-life claims in destination jurisdictions.

Why Auriga for Stability Testing

Unbroken NABL accreditation since 2003

Arbro Group has held NABL ISO/IEC 17025 accreditation continuously since 2003 covering stability assay and microbial trending across the FSSAI 2016 scope.

Walk-in ICH Q1A chambers with continuous monitoring

Multiple walk-in chambers at Zone IVa accelerated, intermediate, long-term, and refrigerated set points — full temperature and humidity audit trail with deviation alarms in YLIMS.

Full assay scope under one roof

Vitamins by HPLC, minerals by ICP-OES, omegas by GC-FID, probiotic CFU, microbial limits, moisture, and physical attributes all run on the same sample at each time point.

ICH Q1B photostability in-house

Validated ICH Option 2 photostability cabinet for vitamins, carotenoids, and fat-soluble actives — no partner-lab dependency for the photostability arm of the dossier.

FSSAI Form B dossier-ready output

Stability reports formatted for direct paste into the FSSAI Form B nutraceutical licence dossier and Form C licence applications — minimises submission revisions.

Five regional labs

Delhi, Gurugram, Bangalore, Baddi, and Bahadurgarh — closer to your factory or warehouse means easier ongoing sample dispatch through the multi-year study window.

Frequently Asked Questions

What is stability testing for nutraceuticals?
Stability testing is the systematic study of how a finished nutraceutical product changes over time under controlled temperature and humidity conditions. It establishes the shelf life (the period during which the product remains within all label claims and safety specifications) and supports the "Best Before" or "Use By" date required on FSSAI-licensed health supplements. Under FSSAI Health Supplements Regulations 2016, stability data is a mandatory component of the Form B nutraceutical licence dossier.
What ICH conditions are used for nutraceutical stability?
Auriga runs stability studies under ICH Q1A(R2) Climate Zone IVa (Hot & Humid) — the climatic zone covering India: Accelerated at 40 °C ± 2 °C / 75% ± 5% RH for 6 months (or short-term 28 days for screening); Intermediate at 30 °C ± 2 °C / 65% ± 5% RH; and Long-term (real-time) at 30 °C ± 2 °C / 75% ± 5% RH for the full claimed shelf life. Refrigerated products (probiotics, fish oil) follow 5 °C ± 3 °C with 25 °C ± 2 °C / 60% ± 5% RH stress conditions where applicable.
What parameters are tested at each stability time point?
Test parameters mirror the release specification: (a) active ingredient assay — every claim-bearing nutrient (vitamins, minerals, omegas, botanical actives, probiotic CFU); (b) microbial limits — TPC, yeast and mould, coliforms, pathogen absence; (c) physical attributes — appearance, colour, friability (tablets), hardness, disintegration; (d) moisture content and water activity (relevant for capsules, powders, sachets); (e) pH and viscosity for liquids. Sampling points typically: 0, 1, 3, 6 months (accelerated); 0, 3, 6, 9, 12, 18, 24 months (long-term).
How long does a stability study take?
A screening accelerated study (28 days) takes 4–6 weeks including all testing rounds. The full 6-month accelerated study takes 7 months elapsed (6 months stability + 4 weeks final testing). A real-time / long-term study matches the claimed shelf life — typically 12 to 36 months for nutraceuticals. Studies can run concurrently, so a 24-month shelf-life claim usually has a 6-month accelerated supplement running in parallel.
What sample quantity is required for stability?
Sample quantity depends on the dosage form, the number of stability time points, and the assay panel. As a working estimate, send 200–300 finished dose units (or 500 g of bulk product) per stability condition in the primary packaging that will be used in market. Stability data is only valid for the specific packaging system tested — different blister foil, sachet film, or bottle materials require independent studies. Our SPOC will confirm the exact quantity after reviewing the protocol.
What FSSAI tolerance applies at end of shelf life?
For nutrients claimed on the label, FSSAI requires the analytical value to be at least 80% of the declared value at end of shelf life. Build a 20–25% overage at manufacture so routine degradation through shelf life still leaves the labelled claim defensible. For probiotics, the same 80% floor applies — typically with a 2–3× overage to absorb CFU loss in storage. Heavy metals and microbial limits must remain within FSSAI limits at every time point, not just at manufacture.
When is a new stability study required?
A new stability study is required whenever: (a) the formulation or excipient composition changes; (b) the active-ingredient supplier or grade changes; (c) the manufacturing site or process changes; (d) the primary packaging (blister foil, sachet film, bottle resin, closure) changes; (e) the claimed shelf life is extended beyond the original supported period. Annual review of long-term stability data is also recommended for products in active commercial production.

Get Your Stability Testing Quote

ICH Q1A Zone IVa stability under accelerated, intermediate, long-term, and refrigerated conditions. NABL-accredited and FSSAI Form B dossier-ready.

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